DNA profiling and PCR Quiz
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5 questions, one answer each
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1. Which stage of the PCR cycle uses a temperature of about 72 °C?
- A. Denaturation
- B. Annealing of primers
- C. Extension of the new strands
- D. Splicing
2. A child has a DNA band that is absent from her mother. What does this show?
- A. The band is a mutation
- B. The band must have been inherited from her father
- C. The mother is not the biological mother
- D. The band is from non-coding DNA only
3. In gel electrophoresis, which statement is correct?
- A. Large fragments travel furthest to the cathode
- B. DNA moves to the anode because phosphate groups are negative
- C. Fragments are separated by their base sequence
- D. The ladder is used to stain the DNA
4. After 8 PCR cycles one DNA molecule has been amplified to how many copies?
- A. 16
- B. 64
- C. 256
- D. 512
5. Two different proteins are made from one gene. Which process best explains this?
- A. Replication of introns
- B. Alternative splicing of the primary transcript
- C. Translation of introns
- D. Reverse transcription