Genetic fingerprintingAQA A-Level Biology: Mind map
What this mind map covers
- VNTRs
- PCR
- Gel electrophoresis
- Interpreting bands
- Uses
Exam questions on Genetic fingerprinting
- Police find a very small bloodstain at the scene of a burglary. A forensic laboratory produces a genetic fingerprint from the DNA in the stain and compares it with the fingerprint of a suspect. The technique analyses variable number tandem repeats (VNTRs), which are short base sequences repeated one after another in non-coding DNA.Explain why two unrelated people are very unlikely to have the same genetic fingerprint.2 marks
- A baby's father is to be identified. DNA from the mother, the child and two men, X and Y, was amplified by PCR and the VNTR fragments were separated by gel electrophoresis. The fragment sizes, in base pairs, were: mother 900, 650, 420 and 310; child 900, 560, 420 and 280; man X 780, 560, 380 and 280; man Y 700, 480, 310 and 200.Use the data to explain which man is the child's father.2 marks
- A zoo breeding programme for an endangered big cat has a small captive population. Scientists use genetic fingerprinting to analyse VNTRs in each animal. They compared two captive populations, X and Y, each of 30 animals, at six VNTR loci. In population X, 24 of the 30 animals have the same VNTR pattern at all six loci. In population Y, 3 of the 30 animals do.Explain why scientists might use genetic fingerprinting in a breeding programme for animals.3 marks
Written by the Exaim team, led by Shaun Daswani (Head of Upper Secondary, Improve ME Institute; MSc Financial Mathematics, Imperial College London; BSc, UCL) and Jason Daswani (operational lead, Improve ME Institute; LSE).