Genetically modified organisms and recombinant DNA

Genetically modified organisms and recombinant DNA Quiz

Spec 8.17–19 · Edexcel International A Level Biology: Quiz

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5 questions, one answer each

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  1. 1. Which enzyme cuts DNA at a specific recognition sequence?

    • A. DNA ligase
    • B. Restriction endonuclease
    • C. DNA polymerase
    • D. Helicase
  2. 2. What bond does DNA ligase form?

    • A. Hydrogen bonds between bases
    • B. Peptide bonds
    • C. Glycosidic bonds between glucose
    • D. Phosphodiester bonds between nucleotides
  3. 3. Why do the sticky ends of a cut plasmid and a cut gene join?

    • A. They are complementary and base pair
    • B. DNA ligase is not needed
    • C. They have identical sequences
    • D. They are held by peptide bonds
  4. 4. A GM tobacco plant is regenerated from leaf cells grown on a medium containing kanamycin. Why is kanamycin used?

    • A. It stimulates cells to divide
    • B. It cuts the plasmid
    • C. It kills cells that did not take up the kanamycin-resistance gene
    • D. It makes the antibody gene work
  5. 5. Why is human antithrombin made in the milk of GM goats rather than in E. coli?

    • A. Goat cells are prokaryotic
    • B. Goat cells, being eukaryotic, can modify a large human protein correctly, and milk is easily harvested
    • C. Goats have no restriction endonucleases
    • D. E. coli cannot use plasmids

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