Monoclonal antibodies and ELISA Quiz
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5 questions, one answer each
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1. A drug is attached to a monoclonal antibody. Why does the drug act mainly on tumour cells?
- A. The drug is only toxic to tumour cells
- B. The antibody's binding site is complementary to an antigen on the tumour cells
- C. The antibody makes healthy cells divide more slowly
- D. The antibody is digested only inside tumour cells
2. In an ELISA test, what causes the colour change?
- A. The first antibody changing shape
- B. The antigen reacting with the substrate
- C. The enzyme on the second antibody converting the substrate
- D. The washing solution
3. Why must wells be washed after the second antibody is added?
- A. To remove unbound enzyme-linked antibody and avoid a false positive
- B. To destroy the antigen
- C. To add more substrate
- D. To make the antibody specific
4. Which is an ethical concern about monoclonal antibodies?
- A. They are identical to each other
- B. Animals such as mice are used to produce them
- C. They bind to a specific antigen
- D. They are proteins
5. Why are monoclonal antibodies used in pregnancy tests rather than a mixture of antibodies?
- A. They bind only hCG, giving fewer false positives
- B. They are cheaper to digest
- C. They change the colour of urine
- D. They stimulate the pregnancy hormone