Recombinant DNA technology Quiz
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5 questions, one answer each
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1. Which enzyme cuts DNA at specific base sequences?
- A. Restriction endonuclease
- B. DNA ligase
- C. DNA polymerase
- D. Reverse transcriptase
2. Why does PCR use a heat-stable DNA polymerase?
- A. It works only at 37 °C
- B. It joins DNA fragments
- C. It makes primers
- D. It is not denatured at about 95 °C
3. In PCR the number of copies after 8 cycles from one molecule is
- A. 16
- B. 64
- C. 256
- D. 512
4. What does a vector do?
- A. Cuts DNA
- B. Carries a gene into the host cell
- C. Makes cDNA
- D. Joins amino acids
5. A student claims bacteria can express a human gene because the genetic code is universal. The student is
- A. wrong, as bacteria use a different code
- B. wrong, as bacteria cannot transcribe
- C. right, as the gene has no promoter
- D. right, as the same triplets code for the same amino acids